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Trypsin 2500U/mg
CAS9002-07-7
Source Swine (Bovine) pancreas
General description
Trypsin is a kind of serine proteolytic enzyme, with molecularweight of 23300 dalton, and is a single peptide chain composed of223 amino acid residues. With rigorous specificity, the trypsinspecifically acts on the peptide linkage constituted by the basicamino acid arginine and leucine. Enzyme easily autolyzes by itself,and its activation also reduces or loses gradually. It is easilysoluble in the water, but insoluble in trichloromethane, ethanol,ether and glycerin, and the optimum PH is 8.0~9.0. When the PH is1.8, it is hardly deactivated when boiled for a short time; if thesalt is added into the hot solution, the protein will precipitate,and the enzyme action of filtrate cannot be seen, and Ca2 plays therole in protecting and activating the trypsin.
The high purity Trypsin of Beijing Geyuantianrun Bio-tech Co.,Ltd.. is purified by re-crystallization, and then byultra-filtration.
Specification
Items | Specification | Method |
Appearance | White crystallized powder | USP33 |
solubility | 500,000 USP Trypsin Unites, is soluble in 10mL of water and in10 mL of saline TS | USP33 |
Loss on drying | ≤ 5.0% | USP33 |
Residue on Ignition | ≤2.5% | USP33 |
Microbial limits |
|
|
Pseudomonas aeruginosa | Absent | USP33 |
Salmonella | Absent | USP33 |
Staphylococcus aureus | Absent | USP33 |
Limit chymotrypsin | NMT 50USP Chymotrypsin Units/2500USP Trypsin Units (NMTapproximately 5% of Chymotrypsin) | USP33 |
Assay | NLT 2500 USP Trypsin Units/mg (calculated on the dried basis)and 90.0%-110.0% of the labeled potency. | USP33 |
Storage
Sealed, Dark, at temperature 2-8°C
Items | Specification | Method |
Description | white crystalline lyophilized powder | EP6.0 |
Identification | A) A purple colour develops B) No colour develops with in 3 min of mixing | EP6.0 |
Appearance of solution | Solution S is not more opalescent than reference suspension 3 | EP6.0 |
Absorbance | 1) 280nm : 13.5 ~ 16.5 2) 250nm : not more than 7.0 | EP6.0 |
PH | 3.0-6.0 | EP6.0 |
Histamine | Not more than 1µg/0.2µkatal | EP6.0 |
Loss on drying | Not more than 5% | EP6.0 |
Limit of trypsin | Not more than 1% | EP6.0 |
Microbial limits Total viable aerobic count Salmonella E Coli |
≤10000cfu/g Negative Negative | EP6.0 |
Assay | Not less than 0.5 ukatal/mg, calculated on the dried basis | EP6.0 |
Storage
Sealed, Dark, at temperature 2-8°C
Trypsin Procine/Bovine pancreashave stock
Trypsin Procine\/Bovine pancreas have stock
